Synthesis and characterization for a highly selective bis(salamo)-based chemical sensor and imaging in living cell

Lan Wang; Zhi-Li Wei; Chang Liu; Wen-Kui Dong; Jia-Xi Ru

doi:10.1016/j.saa.2020.118496

Synthesis and characterization for a highly selective bis(salamo)-based chemical sensor and imaging in living cell

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 5:239:118496. doi: 10.1016/j.saa.2020.118496. Epub 2020 May 16.

Authors

Lan Wang¹, Zhi-Li Wei¹, Chang Liu¹, Wen-Kui Dong², Jia-Xi Ru³

Affiliations

¹ School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China.
² School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China. Electronic address: dongwk@126.com.
³ State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China. Electronic address: rujiaxi@caas.cn.

PMID: 32470813
DOI: 10.1016/j.saa.2020.118496

Abstract

A new sensor H₅L for continuous identification of Cu²⁺, Al³⁺ and lysine was synthesized by Schiff base reactions. The sensor could specifically recognized Cu²⁺ in the EtOH/H₂O (1:1 v/v) solution by UV-vis spectra, and the binding constant with Cu²⁺ can reach 10¹¹ M^-1, meanwhile, it was found by the naked-eye that the color of the solution was changed from colorless to yellow. The copper complex L-Cu²⁺ formed by the sensor H₅L and Cu²⁺ could further recognize Al³⁺ and lysine in the fluorescence spectra. The LOD values of the three objects were 2.67 × 10^-8, 1.96 × 10^-8 and 5.59 × 10^-9 M, respectively. In addition, fluorescence intracellular images of Al³⁺ and lysine were performed and obtained satisfactory results.

Keywords: Bis(salamo)-based fluorescent probe; Cell imaging; Copper complex; Relay recognition.

MeSH terms

Copper
Fluorescent Dyes*
Schiff Bases*
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Schiff Bases
Copper