Enhanced healing of rat calvarial defects with 3D printed calcium-deficient hydroxyapatite/collagen/bone morphogenetic protein 2 scaffolds

Shi Huan Han; JiUn Lee; Kyung Mee Lee; Yuan Zhe Jin; Hui-Suk Yun; GeunHyung Kim; Jae Hyup Lee

doi:10.1016/j.jmbbm.2020.103782

Enhanced healing of rat calvarial defects with 3D printed calcium-deficient hydroxyapatite/collagen/bone morphogenetic protein 2 scaffolds

J Mech Behav Biomed Mater. 2020 Aug:108:103782. doi: 10.1016/j.jmbbm.2020.103782. Epub 2020 Apr 16.

Authors

Shi Huan Han¹, JiUn Lee², Kyung Mee Lee³, Yuan Zhe Jin⁴, Hui-Suk Yun⁵, GeunHyung Kim⁶, Jae Hyup Lee⁷

Affiliations

¹ Department of Orthopedic Surgery, College of Medicine, Seoul National University, Seoul, 03080, South Korea; Department of Orthopedic Surgery, YanBian University Hospital, 133000, Yanji, Jilin Province, China.
² Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.
³ Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul, 07061, South Korea.
⁴ Department of Orthopedic Surgery, The First Hospital of Jilin University, 130000, Changchun, Jilin Province, China.
⁵ Department of Advanced Biomaterials Research, Materials Processing Innovation Research Division, Korea Institute of Materials Science (KIMS), Changwon, South Korea.
⁶ Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea. Electronic address: gkimbme@skku.edu.
⁷ Department of Orthopedic Surgery, College of Medicine, Seoul National University, Seoul, 03080, South Korea; Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul, 07061, South Korea; Institute of Medical and Biological Engineering, Seoul National University, Seoul, 110-799, South Korea. Electronic address: spinelee@snu.ac.kr.

PMID: 32469715
DOI: 10.1016/j.jmbbm.2020.103782

Abstract

In this paper, we mainly to evaluate the newly formed bone using the Calcium deficient hydroxyapatite (CDHA)/collagen-based bio-ceramic scaffold as Bone Morphogenetic Protein-2 (BMP-2) carrier in rat calvarial critical-sized bone defect. In the real-time PCR analysis, the CDHA/collagen scaffold loaded rhBMP-2 group showed significantly enhanced results of bone-related gene expression (p < 0.05). In the in vivo study, the micro-CT showed that the main bone formation parameters of percent bone volume and trabecular number of the two experiment groups (CDHA/Collagen (CDHA) group, BV/TV: 14.21 ± 3.20, Tb.N: 2.37 ± 0.50; CDHA/Collagen/rhBMP-2(BMP) group, BV/TV: 14.51 ± 3.12, Tb.N: 2.75 ± 0.65) were significantly higher than those of the control (Blank, BV/TV: 3.25 ± 1.25, Tb.N: 0.57 ± 0.20) group (p < 0.05). Although there was no significant difference between the two experimental groups, the BMP group results were slightly higher than those of the CDHA group (p > 0.05). Moreover, the histological results also supported the micro-CT results. The scaffold of CDHA/collagen seems to be a suitable bio-ceramic carrier loaded rhBMP-2, and appears to enhance new bone formation and bone regeneration in bone defect after implantation.

Keywords: Bone morphogenetic Protein-2; Calcium deficient hydroxyapatite; Calvarial bone defect; Collagen; In vivo study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Morphogenetic Protein 2*
Bone Regeneration
Calcium*
Collagen
Printing, Three-Dimensional
Rats
Skull / diagnostic imaging

Substances

Bone Morphogenetic Protein 2
Collagen
Calcium