Living Filtration Membranes (LFMs) are a water filtration technology that was recently developed in the lab (Technology Readiness Level 4). LFMs have shown filtration performance comparable with that of ultrafiltration, far better fouling resistance than conventional polymer membranes, and good healing capabilities. These properties give LFMs promise to address two significant issues in conventional membrane filtration: fouling and membrane damage. To integrate environmental considerations into future technology development (i.e., Ecodesign), this study assesses the life cycle environmental performance of drinking water treatment using LFMs under likely design and operation conditions. It also quantitatively ranks the engineering design and operation factors governing the further optimization of LFM environmental performance using a global sensitivity analysis. The results suggest that LFMs' superior fouling resistance will reduce the life cycle environmental impacts of ultrafiltration by 25% compared to those of a conventional polymer membrane in most impact categories (e.g., acidification, global warming potential, and carcinogenics). The only exception is the eutrophication impact, where the need for growth medium and membrane regeneration offsets the benefits of LFMs' fouling resistance. Permeability is the most important factor that should be prioritized in future R&D to further improve the life cycle environmental performance of LFMs. A 1% improvement in the permeability will lead to a ∼0.7% improvement in LFMs' environmental performance in all the impact categories, whereas the same change in the other parameters investigated (e.g., LFM lifespan and regeneration frequency) typically only leads to a <0.2% improvement.