Presence of viral RNA of SARS-CoV-2 in conjunctival swab specimens of COVID-19 patients

Indian J Ophthalmol. 2020 Jun;68(6):1015-1017. doi: 10.4103/ijo.IJO_1287_20.

Abstract

Purpose: To detect the presence of viral RNA of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in conjunctival swab specimens of coronavirus disease-19 (COVID-19) patients.

Methods: Forty-five COVID-19 patients positive for real-time reverse transcription-polymerase chain reaction (RT-PCR) for SARS-CoV-2 in nasopharyngeal swab with or without ocular manifestations were included in the study. The conjunctival swab of each patient was collected by an ophthalmologist posted for COVID duty.

Results: Out of 45 patients, 35 (77.77%) were males and the rest were females. The mean age was 31.26 ± 12.81 years. None of the patients had any ocular manifestations. One (2.23%) out of 45 patients was positive for RT-PCR SARS-CoV-2 in the conjunctival swab.

Conclusion: This study shows that SARS-CoV-2 can be detected in conjunctival swabs of confirmed cases of COVID-19 patients. Though the positivity rate of detecting SARS-CoV-2 in conjunctival swabs is very less, care should be exercised during the ocular examination of patients of COVID-19.

Keywords: Conjunctival swab; SARS-CoV-2; coronavirus disease-19; nasopharyngeal swab.

MeSH terms

  • Adolescent
  • Adult
  • Betacoronavirus / genetics*
  • COVID-19
  • COVID-19 Testing
  • COVID-19 Vaccines
  • Child
  • Clinical Laboratory Techniques
  • Conjunctivitis, Viral / diagnosis*
  • Coronavirus Infections / diagnosis*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nasal Cavity / virology
  • Pandemics
  • Pharynx / virology
  • Pneumonia, Viral / diagnosis*
  • Prospective Studies
  • RNA, Viral / isolation & purification*
  • Real-Time Polymerase Chain Reaction
  • SARS-CoV-2
  • Specimen Handling
  • Young Adult

Substances

  • COVID-19 Vaccines
  • Covid-19 aAPC vaccine
  • RNA, Viral