Objective: To investigate the clinical characteristics and the distribution of peripheral blood T lymphocyte sub-sets in patients with schistosomal hepatic cirrhosis in Suzhou City.
Methods: A total of 32 inpatients with liver diseases due to advanced schistosomiasis at the Department of Infectious Diseases, The First Affiliated Hospital of Soochow University from January 2016 to January 2018 were recruited and assigned into the infection and non-infection groups according to presence of co-infections, and 20 old healthy volunteers served as controls. Venous blood samples were collected on the day of admission, and the proportions of CD4+ T cells, CD8+ T cells, regulatory T (Treg) cells and Th17 cells were detected in peripheral blood using flow cytometry.
Results: Most patients with liver disorders due to advanced schistosomiasis were admitted to hospital in Suzhou City because of portal hypertension-associated complications, with a high prevalence of co-infections (59.38%, 19/32). The proportions of peripheral CD4+ and CD8+ T cells and Th17 cells were all significantly lower in patients with liver disorders due to advanced schistosomiasis than in controls (t = -5.111, -4.470 and -2.749, all P < 0.05), and a higher proportion of Treg cells was detected in patients than in controls (t = 5.628, P < 0.05). In addition, there were significant differences among the infection group, non-infection group and controls in terms of the percentage of CD4+ T cells, CD8+ T cells, Th17 cells and Treg cells (F = 15.837, 16.594, 9.290 and 27.866, all P < 0.05).
Conclusions: Portal hypertension-associated complications are predominantly seen in patients with liver diseases due to advanced schistosomiasis at admission in Suzhou City, and co-infections are common. Imbalance of peripheral T cell subsets is detected in patients with liver diseases due to advanced schistosomiasis in Suzhou City.
［摘要］ 目的 了解苏州地区晚期血吸虫病肝病患者临床特征及其外周血T淋巴细胞亚群分布。方法 以 2016 年 1 月-2018年 1 月在苏州大学附属第一医院感染科住院治疗的 32 例晚期血吸虫病肝病患者为研究对象, 并根据是否合并 其他感染分组进行研究; 同时随机选取 20 例老年健康体检者为对照。采集研究对象静脉血, 采用流式细胞术检测外周 血 CD4+T 细胞、CD8+T 细胞、调节性T (Treg) 细胞和Th17细胞比例。结果 苏州地区血吸虫性肝病患者多因门脉高压相 关并发症入院, 合并感染率较高 (59.38%, 19/32)。血吸虫性肝病患者外周血 CD4+T、CD8+T 淋巴细胞及Th17细胞百分比 低于健康对照组 (t = −5.111、−4.470 和 −2.749, P 均＜ 0.05); Treg 细胞百分比高于健康对照组 (t = 5.628, P ＜ 0.05)。有感 染组、无感染组及对照组患者的 CD4+T、CD8+T 淋巴细胞、Th17 细胞及Treg 细胞亚群分布差异均有统计学意义 (F = 15.837、16.594、9.290 和 27.866, P 均＜ 0.05)。结论 苏州地区血吸虫性肝病患者入院多以门脉高压相关并发症多见, 合 并感染者较常见, 且患者外周血T淋巴细胞亚群已处于失衡状态。.
Keywords: Clinical characteristic; Schistosomal liver disease; Suzhou City; T lymphocyte subsets.