The present study aims to perform comparative genetic characterization for R. sanguineus s.l. infesting Algerian dogs living in steppe and high plateau regions based on mitochondrial 16S rRNA gene sequencing and phylogenetic analysis. In total, 1043 ticks were collected from 147 infested dogs, including 756 ticks from 124 dogs in Djelfa and 287 ticks from 23 dogs in Bordj-Bou-Arreridj. All ticks were morphologically identified as R. sanguineus s.l., and 87 specimens were randomly selected for molecular analysis. Sequences obtained from selected ticks clustered with sequences of R. sanguineus s.l. from France, Germany, Portugal, and Spain, all of which belong to R. sanguineus s.s. The occurrence of R. sanguineus s.s. in Algeria is an interesting result given that most R. sanguineus in Africa cluster among the tropical lineage. This finding should prompt further investigation by exhaustive studies across Algerian territory. Such data can be useful to understand the dispersal route of R. sanguineus s.s. from Europe to North Africa, or whether their occurrence in Algeria is a result of historical biogeography.
Keywords: Algeria; Dogs; Rhipicephalus sanguineus sensu stricto; Ticks.