SpRY: Engineered CRISPR/Cas9 Harnesses New Genome-Editing Power

Dangquan Zhang; Baohong Zhang

doi:10.1016/j.tig.2020.05.001

SpRY: Engineered CRISPR/Cas9 Harnesses New Genome-Editing Power

Trends Genet. 2020 Aug;36(8):546-548. doi: 10.1016/j.tig.2020.05.001. Epub 2020 May 23.

Authors

Dangquan Zhang¹, Baohong Zhang²

Affiliations

¹ Henan Province Engineering Research Center for Forest Biomass Value-Added Products, College of Forestry, Henan Agricultural University, Zhengzhou, Henan 450002, China. Electronic address: zhangdangquan@163.com.
² Department of Biology, East Carolina University, Greenville, NC 27858, USA. Electronic address: zhangb@ecu.edu.

PMID: 32456805
DOI: 10.1016/j.tig.2020.05.001

Abstract

Due to protospacer adjacent motif (PAM) requirements, CRISPR/Cas9 cannot access many genetic loci. A recent study by Walton et al. structurally engineered Streptococcus pyogenes Cas9 (SpCas9) to near-PAMless SpRY that can target most DNA sequences with high editing efficiency and flexibility. This newly engineered SpRY will potentially expand genome-editing capabilities for basic and applied research.

Keywords: CRISPR/Cas9; PAM requirement; SpRY; gene function study; genome editing.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Comment

MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats
DNA
Gene Editing*
Streptococcus pyogenes / genetics

Substances

DNA