Are concentrations of clusterin and beta-2-glycoprotein I dysregulated in HIV associated preeclampsia?

Abstract

Objective: To evaluate the levels of serum beta-2-glycoprotein I (β₂GP1) and clusterin in the duality of Pre-eclampsia and HIV.

Method: Stored serum samples collected from 72 pregnant women were stratified according to the pregnancy type (pre-eclamptic and healthy normotensive groups) and HIV status (positive or negative). A Bio-Plex multiplex immunoassay was used to determine the concentrations of clusterin and β₂GP1.

Results: Clusterin concentrations differed significantly (p = 0.01) between the HIV positive (+) (mean = 123 800 ng/ml; 95 % CI: 105 400-142 200) vs. HIV negative (-) (mean = 92 190 ng /ml; 95 %CI: 75 840-108 500) groups and across all groups (p = 0.0006). Beta-2-glycoprotein I concentration differed significantly based on HIV status (p < 0.0001); HIV+ (mean = 393 649 ng/ml; 95 %CI: 30 300-467 000) vs HIV- (mean = 224 309 ng/ml; 95 %CI: 154 000-294 700) and across all groups (p < 0.0001). No significant difference was observed between normotensive and Pre-eclamptic groups for both clusterin and β₂GPI.

Conclusion: Serum concentrations of clusterin and β₂GPI were significantly increased in HIV positive pregnancies. It is postulated that both clusterin and β₂GPI may have a role in HIV disease progression. These findings need to be confirmed in studies having larger sample sizes and detailed information on anti-retroviral therapy.

Keywords: Clusterin; HIV; Pre-eclampsia; β(2)GPI.