Identification of Transposable Elements in Schistosoma mansoni

Methods Mol Biol. 2020:2151:135-144. doi: 10.1007/978-1-0716-0635-3_11.

Abstract

Transposable elements (TEs) represent a significant portion of eukaryotic genomes and are important players in their dynamics and evolution. Therefore, the description of TEs and the analysis of their distribution in the genomes are important steps to understand their influence in the architecture of genomes. Here we describe the protocol used by us to identify and curate consensus TEs sequences from S. mansoni, as well the protocol to map these elements in the S. mansoni genome. We expect that these protocols may help researchers interested in studying TEs content in S. mansoni or other organisms.

Keywords: Bioinformatics analyses; Copies annotation; Genome; Schistosoma mansoni; Transposable elements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Transposable Elements / genetics*
  • Genome
  • Molecular Sequence Annotation
  • Schistosoma mansoni / genetics*

Substances

  • DNA Transposable Elements