Photo-fermentation biohydrogen production and electrons distribution from dark fermentation effluents under batch, semi-continuous and continuous modes

Yameng Li; Zhiping Zhang; Chenxi Xia; Yanyan Jing; Quanguo Zhang; Suiliang Li; Shengnan Zhu; Peng Jin

doi:10.1016/j.biortech.2020.123549

Photo-fermentation biohydrogen production and electrons distribution from dark fermentation effluents under batch, semi-continuous and continuous modes

Bioresour Technol. 2020 Sep:311:123549. doi: 10.1016/j.biortech.2020.123549. Epub 2020 May 19.

Authors

Yameng Li¹, Zhiping Zhang¹, Chenxi Xia², Yanyan Jing³, Quanguo Zhang⁴, Suiliang Li¹, Shengnan Zhu⁵, Peng Jin¹

Affiliations

¹ Key Laboratory of New Materials and Facilities for Rural Renewable Energy MOA of China, Henan Agricultural University, Zhengzhou 450002, China.
² Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China; Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Zhengzhou 450002, China.
³ Key Laboratory of New Materials and Facilities for Rural Renewable Energy MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Zhengzhou 450002, China.
⁴ Key Laboratory of New Materials and Facilities for Rural Renewable Energy MOA of China, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: zquanguo@163.com.
⁵ Key Laboratory of New Materials and Facilities for Rural Renewable Energy MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.

PMID: 32451140
DOI: 10.1016/j.biortech.2020.123549

Abstract

In this work, the influence of batch, semi-continuous and continuous mode on biohydrogen production from dark fermentation effluents (DFEs) as substrate and electron distribution was investigated. Results indicated a better H₂ production performance was obtained in semi-continuous mode. 50% decanting volume ratio (DVR) and 24 h feeding interval time (FIT) were found to be the best condition. Maximum average H₂ production rate (HPR) and H₂ yield were obtained of 8.44 mL/h and 1386.22 ± 44.23 mL H₂/g TOC, respectively. 37.71% substrate electrons partitioning to hydrogen were detected. For continuous mode, more substrate electrons were diverted toward SMPs with the increasing of HRT due to the fact that longer cell retention, more chances were provided for cell lysis. The bad performance in batch mode ascribed to 56.39% substrate electrons were transferred to cell growth and soluble microbial products (SMPs).

Keywords: Batch fermentation; Biohydrogen; Continuous fermentation; Electrons distribution; Semi-continuous fermentation.

MeSH terms

Bioreactors
Electrons*
Fermentation
Hydrogen*
Hydrogen-Ion Concentration
Longitudinal Studies

Substances

Hydrogen