Pharmacokinetic profile of diosgenin and trigonelline following intravenous and oral administration of fenugreek seed extract and pure compound in rabbit

Abstract

Diosgenin (DI) and trigonelline (TRG) are the main bioactive compounds of fenugreek seed. A micro-extraction method using magnetic nanocomposites coupled with ion-pairing reversed phase HPLC (RP-HPLC) method was used to determine the analytes in rabbit plasma. The main PK parameters after oral administration of pure DI and the extract were: C_max: 0.48, 0.057 µg/ml; t_max: 1, 2 h; and t_1/2β: 6.23, 15.04 h, respectively. Pure TRG and the extract PK parameters were: C_max: 0.121, 0.081 µg/ml; t_max: 1.30, 1.30 h; and t_1/2β: 20.06, 24.70 h, respectively. Generally, the PK profile of the two compounds was best fitted on the two-compartment model.[Formula: see text].

Keywords: magnetic nanoparticles; pharmacokinetics; Fenugreek; diosgenin; trigonelline.