Simultaneous analysis of polyethylene glycol in dietary supplement tablets using high-performance liquid chromatography and verification with liquid chromatography-electrospray ionization mass spectrometry

Hyondeog Kim; Juhee Park; Hee-Jae Suh; Jihyun Lee; Chan Lee

doi:10.1016/j.foodchem.2020.126933

Simultaneous analysis of polyethylene glycol in dietary supplement tablets using high-performance liquid chromatography and verification with liquid chromatography-electrospray ionization mass spectrometry

Food Chem. 2020 Oct 1:326:126933. doi: 10.1016/j.foodchem.2020.126933. Epub 2020 Apr 27.

Authors

Hyondeog Kim¹, Juhee Park², Hee-Jae Suh³, Jihyun Lee⁴, Chan Lee⁵

Affiliations

¹ Advanced Food Safety Research Group, BrainKorea21 Plus, Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea; Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea. Electronic address: hdyj5359@naver.com.
² Advanced Food Safety Research Group, BrainKorea21 Plus, Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea; Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea. Electronic address: bjhwngml@naver.com.
³ Department of Food Science, Sun Moon University, Asan, Chungchengnam-do 31460, Republic of Korea. Electronic address: suhhj@sunmoon.ac.kr.
⁴ Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea. Electronic address: jihlee@cau.ac.kr.
⁵ Advanced Food Safety Research Group, BrainKorea21 Plus, Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea; Department of Food Science and Technology, Chung-Ang University, 4726, Seodong-daero, Anseong, Gyeonggi-do 17546, Republic of Korea. Electronic address: chanlee@cau.ac.kr.

PMID: 32438228
DOI: 10.1016/j.foodchem.2020.126933

Abstract

Polyethylene glycol (PEG) are widely applied in detergents, cosmetics, and food additives. A simultaneous analytical method was developed to detection the polyethylene glycol (100-10000 Da). High-performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) could analyze successively PEG products. The method was verified with liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS). The retention times of PEG 200-8000 ranged from 1.97 to 12.33 min. Method validation was performed to the International Conference on Harmonization (ICH) guidelines and the Korea Ministry of Food and Drug Safety (MFDS); linearity: R² > 0.997, LOD: 7.47-16.24 µg/mL, LOQ: 22.40-75 µg/mL, repeatability (%RSD): 0.2-2.5, recovery (%): 90.4-104.9% for film-coated tablet, 80.1-95.9% for sugar-coated tablet. A total of 115 PEG could be identified by extracted ion chromatography in mass analysis, based on the charge state represented as [M+Na⁺+H₃O⁺_n-1]. This method can be applied for successive identification of PEGs in PEG-containing products.

Keywords: Food coating agent; High-performance liquid chromatography analysis; Liquid chromatography tandem mass spectrometry; Polyethylene glycol; Polyethylene glycol (PubChem CID 174).

Publication types

Validation Study

MeSH terms

Chromatography, High Pressure Liquid / methods*
Dietary Supplements / analysis*
Polyethylene Glycols / analysis*
Republic of Korea
Spectrometry, Mass, Electrospray Ionization / methods*
Tablets / analysis
Tandem Mass Spectrometry / methods

Substances

Tablets
Polyethylene Glycols