Use of the HRM Method in Quick Identification of FecXO Mutation in Highly Prolific Olkuska Sheep

Grzegorz Smołucha; Katarzyna Piórkowska; Katarzyna Ropka-Molik; Jacek Sikora

doi:10.3390/ani10050844

Use of the HRM Method in Quick Identification of FecX^O Mutation in Highly Prolific Olkuska Sheep

Animals (Basel). 2020 May 14;10(5):844. doi: 10.3390/ani10050844.

Authors

Grzegorz Smołucha¹, Katarzyna Piórkowska¹, Katarzyna Ropka-Molik¹, Jacek Sikora²

Affiliations

¹ Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
² Department of Sheep and Goat Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.

Abstract

Olkuska is a highly prolific sheep breed in Poland. Thanks to earlier identification of the genetic basis of its prolificacy, a mutation in the BMP-15 gene, we can use molecular biology tools to identify this causative mutation affecting prolificacy. In our research, we used the High-Resolution Melting (HRM) and Sanger sequencing methods to identify the genotypes of the studied animals. The result obtained by the HRM method is identical to those obtained by the sequencing method, which confirms the effectiveness of the HRM method and the possibility of quick and cheap identification of individuals with a FecX^O mutation.

Keywords: BMP-15; HRM; Olkuska; prolificacy; sheep.

Grants and funding

/297267/14/NCBR/2016/BIOSTRATEG2