Production of plant natural products through engineered Yarrowia lipolytica

Akram Muhammad; Xudong Feng; Aamir Rasool; Wentao Sun; Chun Li

doi:10.1016/j.biotechadv.2020.107555

Production of plant natural products through engineered Yarrowia lipolytica

Biotechnol Adv. 2020 Nov 1:43:107555. doi: 10.1016/j.biotechadv.2020.107555. Epub 2020 May 15.

Authors

Akram Muhammad¹, Xudong Feng², Aamir Rasool¹, Wentao Sun¹, Chun Li³

Affiliations

¹ Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
² Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China. Electronic address: xd.feng@bit.edu.cn.
³ Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China. Electronic address: lichun@bit.edu.cn.

PMID: 32422161
DOI: 10.1016/j.biotechadv.2020.107555

Abstract

Plant natural products have broad applications in the pharmaceutical, food, cosmetics and flavor industries. However, the current manufacturing methods for plant natural products mainly rely on plant extraction and chemical synthesis, which are associated with unsustainability and severe environmental problems. The advancement of genetic technology has facilitated the engineering of microbes for producing plant natural products, but their industrial production titers remain low. Yarrowia lipolytica, a nonconventional yeast, is a promising and superior host for plant natural products manufacture, due to its advanced properties compared to conventional hosts. In this review, metabolic engineering approaches recently employed for overproduction of useful plant natural products, including terpenoids and polyketides, in Y. lipolytica are summarized. The current challenges for the biosynthesis of typical compounds are discussed and promising engineering strategies for future work are also suggested. This review provides guidance for engineering Y. lipolytica as a robust host for plant natural products production.

Keywords: Flavonoids; Metabolic engineering; Natural products; Polyketides; Terpenoids; Yarrowia lipolytica.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biological Products*
Metabolic Engineering
Polyketides*
Terpenes
Yarrowia* / genetics

Substances

Biological Products
Polyketides
Terpenes