Dual-color STED super-resolution microscope using a single laser source

Jialin Wang; Jia Zhang; Luwei Wang; Xinwei Gao; Yonghong Shao; Liwei Liu; Zhigang Yang; Wei Yan; Junle Qu

doi:10.1002/jbio.202000057

Dual-color STED super-resolution microscope using a single laser source

J Biophotonics. 2020 Aug;13(8):e202000057. doi: 10.1002/jbio.202000057. Epub 2020 Jun 5.

Authors

Jialin Wang¹, Jia Zhang¹, Luwei Wang¹, Xinwei Gao¹, Yonghong Shao¹, Liwei Liu¹, Zhigang Yang¹, Wei Yan¹, Junle Qu¹

Affiliation

¹ Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, China.

PMID: 32421923
DOI: 10.1002/jbio.202000057

Abstract

STED (stimulated emission depletion) microscopy is one of the most promising super-resolution fluorescence microscopies，due to its fast imaging and ultra-high resolution. In this paper, we present a dual-color STED microscope with a single laser source. Polarization beam splitters are used to separate the output from a supercontinuum laser source into four laser beams, including two excitation beams (488, 635 nm) and two depletion beams (592, 775 nm). These four laser beams are then used to build a low cost dual-color STED system to achieve a spatial resolution of 75 nm in cell samples.

Keywords: STED microscopy; cell imaging; dual-color imaging; super-resolution; supercontinuum laser.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Lasers*
Microscopy, Fluorescence