Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis

Yongcang Cao; Xiaodong Liang; Changyi Li; Tao Chen; Zhanling Li; Wanfeng Li; Peipei Liu; Guiyong Li; Ran Ma; Yingxue Tang

doi:10.1155/2020/7027391

Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis

Evid Based Complement Alternat Med. 2020 Apr 26:2020:7027391. doi: 10.1155/2020/7027391. eCollection 2020.

Authors

Yongcang Cao^{1

2}, Xiaodong Liang¹, Changyi Li³, Tao Chen², Zhanling Li², Wanfeng Li², Peipei Liu², Guiyong Li², Ran Ma¹, Yingxue Tang¹

Affiliations

¹ Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, Shandong, China.
² Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.
³ China Resources Shandong Pharmaceutical Co., Ltd., 1088 Meili Road, Jinan 250000, Shandong, China.

Abstract

Objective: To investigate the intervention effect and mechanism of Aconite and Angelica sinensis on myocardial ischemia rats with Yang deficiency and blood stasis.

Methods: SPF-class SD rats were randomly divided into low-dose and high-dose groups. Each group was divided into control group, model group, and drug-administered group (FZ, DG, FG; 1 : 0.5, 1 : 1, 1 : 2). A rat model was prepared by intraperitoneal injection of hydrocortisone and isoproterenol plus cold stimulation. Each group was given corresponding decoction or distilled water for 14 days. The behavioral changes of rats in each group were observed. The morphological changes of rats cardiomyocytes were observed by HE staining. The average optical density (MOD value) and percentage of positive cells of Bcl-2, Bax, and Akt were determined by immunohistochemical staining method, and PEIs were calculated. Western blot and RT-PCR were used to determine the expression of PI3K, Caspase-3, Akt protein, and gene expression.

Results: The compatibility of Aconite and Angelica sinensis improved the morphology of rat cardiomyocytes, increased the PEI values of Akt and Bcl-2 protein, and decreased the PEI values of Bax protein (P < 0.01). The compatibility reduced the expression of Caspase-3 protein of rat myocardium and increased the protein expression of p-Akt, PI3K, and p-PI3K (P < 0.01). The compatibility also significantly reduced the expression of Caspase-3 mRNA and increased the expression of PI3K mRNA and Akt mRNA (P < 0.05 or P < 0.01), and the effect of high-dose FG (1 : 2) group is the best.

Conclusions: The method of preparing a rat model of myocardial ischemia with Yang deficiency and blood stasis was feasible. The compatibility of Aconite and Angelica sinensis reduced myocardial fibrosis and inflammatory reaction, protected ischemic cardiomyocytes, and reduced myocardial injury, whose mechanism may be related to the regulation of PI3K/Akt pathway. The compatible group had better intervention effects than Aconite or Angelica sinensis alone. The best one was high-dose FG (1 : 2).