Rewired Cas9s with Minimal Sequence Constraints

Sébastien Levesque; Daniel Agudelo; Yannick Doyon

doi:10.1016/j.tips.2020.04.009

Rewired Cas9s with Minimal Sequence Constraints

Trends Pharmacol Sci. 2020 Jul;41(7):429-431. doi: 10.1016/j.tips.2020.04.009. Epub 2020 May 13.

Authors

Sébastien Levesque¹, Daniel Agudelo¹, Yannick Doyon²

Affiliations

¹ Centre Hospitalier Universitaire de Québec Research Center-Université Laval, Québec, QC G1V 4G2, Canada.
² Centre Hospitalier Universitaire de Québec Research Center-Université Laval, Québec, QC G1V 4G2, Canada. Electronic address: Yannick.Doyon@crchudequebec.ulaval.ca.

PMID: 32416935
DOI: 10.1016/j.tips.2020.04.009

Abstract

The genome editing toolkit is ever expanding. Although CRISPR-Cas systems can target virtually any gene, single-nucleotide resolution is yet to be achieved. Walton and colleagues engineered nucleases and base editors compatible with every protospacer adjacent motif (PAM) to achieve high-precision targeting. Their findings revealed the striking plasticity of Cas9.

Keywords: CRISPR-Cas9; genome editing; protospacer adjacent motifs.

Publication types

Research Support, Non-U.S. Gov't
Comment

MeSH terms

CRISPR-Cas Systems* / genetics
Clustered Regularly Interspaced Short Palindromic Repeats*
Gene Editing