Isotope Labeling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis

Methods Mol Biol. 2020:2146:213-222. doi: 10.1007/978-1-0716-0603-2_16.

Abstract

Isotope labeling enables the detection and quantification of nutrient fluxes between soil and plants through arbuscular mycorrhizal (AM) fungi. Here we describe the use of radioactive isotopes, 33P and 32P, to study the uptake of P from soil by AM fungal mycelium and its transfer to the host plant through the mycorrhizal pathway.

Keywords: Arbuscular mycorrhizal fungi; Phosphorus; Radioactive; Tracer; Uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Isotope Labeling / methods*
  • Mycorrhizae / isolation & purification
  • Mycorrhizae / metabolism*
  • Phosphorus / metabolism*
  • Plant Roots / microbiology
  • Plant Shoots / microbiology
  • Soil Microbiology
  • Symbiosis / genetics*

Substances

  • Phosphorus