Conjugation of Synthetic Trisaccharide of Staphylococcus aureus Type 8 Capsular Polysaccharide Elicits Antibodies Recognizing Intact Bacterium

Ming Zhao; Chunjun Qin; Lingxin Li; Haotian Xie; Beining Ma; Ziru Zhou; Jian Yin; Jing Hu

doi:10.3389/fchem.2020.00258

Conjugation of Synthetic Trisaccharide of Staphylococcus aureus Type 8 Capsular Polysaccharide Elicits Antibodies Recognizing Intact Bacterium

Front Chem. 2020 Apr 28:8:258. doi: 10.3389/fchem.2020.00258. eCollection 2020.

Authors

Ming Zhao¹, Chunjun Qin¹, Lingxin Li¹, Haotian Xie², Beining Ma², Ziru Zhou², Jian Yin¹, Jing Hu²

Affiliations

¹ Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
² Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Abstract

Staphylococcus aureus causes a wide range of life-threatening diseases. One of the powerful approaches for prevention and treatment is to develop an efficient vaccine as antibiotic resistance greatly increases. S. aureus type 8 capsular polysaccharide (CP8) has shown great potential in vaccine development. An understanding of the immunogenicity of CP8 trisaccharide repeating unit is valuable for epitope-focused vaccine design and cost-efficient vaccine production. We report the chemical synthesis of conjugation-ready CP8 trisaccharide 1 bearing an amine linker, which effectively served for immunological evaluation. The trisaccharide 1-CRM197 conjugate elicited a robust immunoglobulin G (IgG) immune response in mice. Both serum antibodies and prepared monoclonal antibodies recognized S. aureus strain, demonstrating that synthetic trisaccharide 1 can be an efficient antigen for vaccine development.

Keywords: Staphylococcus aureus; capsular polysaccharide; carbohydrate-based vaccine; immunogenicity; immunoglobulin G (IgG); monoclonal antibody; synthetic oligosaccharide.