Cryptosporidium spp surveillance and epidemiology in Ireland: a longitudinal cohort study employing duplex real-time PCR based speciation of clinical cases

J Clin Pathol. 2020 Nov;73(11):758-761. doi: 10.1136/jclinpath-2020-206479. Epub 2020 May 14.

Abstract

Cryptosporidium is a leading cause of gastroenteritis (cryptosporidiosis), with significant morbidity and mortality worldwide. Irish cryptosporidiosis incidence rates are consistently the highest reported in Europe. A retrospective, longitudinal study of clinical Cryptosporidium isolates was conducted from 2015 to 2018 in Cork, southern Ireland. Overall, 86.5% of cases were attributed to C. parvum, while the remaining 13.5% were caused by C. hominis Despite the widespread implications of this protozoan parasite in sporadic and outbreak-related illness in Ireland, the current dearth of species-level epidemiological surveillance and clinical studies needs to be addressed in order to elucidate the national impact of this enteric pathogen.

Keywords: PCR; cryptosporidium; epidemiology; molecular biology; parasites.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Child, Preschool
  • Cohort Studies
  • Cryptosporidiosis / epidemiology*
  • Cryptosporidiosis / parasitology
  • Cryptosporidium / genetics
  • Cryptosporidium / isolation & purification*
  • Epidemiological Monitoring
  • Feces / parasitology
  • Female
  • Gastroenteritis / epidemiology*
  • Gastroenteritis / parasitology
  • Humans
  • Ireland / epidemiology
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Real-Time Polymerase Chain Reaction
  • Retrospective Studies
  • Young Adult