Bioconversion of bamboo shoot shell to methane assisted by microwave irradiation and fungus metabolism

Abstract

Bamboo shoot shell (BSS), a major byproduct from bamboo shoot industries with a high amount of output annually, needs to be sustainably management due to its impact on environment and human health. Anaerobic digestion is an eco-friendly and sustainable option, but its efficiency is limited by recalcitrance of lignocellulose structure. A cascade pretreatment (CP) using microwave irradiation and fungus metabolism was developed in this work to reduce the recalcitrance of BSS and enhance its methane production. The results showed significant synergistic effects of microwave irradiation and fungus metabolism on anaerobic digestion of BBS. The methane yield by CP increased by 162.9% (reached to 223.4 mL/g VS) when compared to control group. This was higher than both the values of fungal pretreatment (101.0 mL/g VS, 18.9% increase), and microwave pretreatment (110.5 mL/g VS, 30.1% increase) alone. Further mechanisms of the synergistic effects were revealed. Microwave irradiation provided dissolved products and more accessible BBS for fungus action. In particular, the GC-MS analysis indicated the dissolved products induced fungal laccase activity effectively, and the highest activity in CP was 1.91-fold higher than that in fungal pretreatment alone. The fungus in cascade process further increased accessible surface area and reducing sugars (20.2-43.2%, which compared to fungal pretreatment alone), and reduced significantly the lignin content (42.2-49.1%) and crystallinity (4.5-8.1%) of BSS.

Keywords: Bamboo shoot shell; Cascade pretreatment; Fungus metabolism; Methane production; Microwave irradiation; Synergistic effects.