L-glutamine as a T2 exchange contrast agent

Abstract

Purpose: The potential of L-glutamine as a T₂ exchange contrast agent in MRI was investigated.

Methods: The T₂ relaxation rate of L-glutamine solutions prepared in various concentrations was measured at 9.4 T. A series of T₂ -weighted images in a mouse cancer model was acquired with an L-glutamine solution infusion.

Results: The T₂ relaxivity caused by the exchange (R_2ex ) at 37°C was 0.069 s^-1 mM^-1 and 0.102 s^-1 mM^-1 for glutamine and glutamate solutions at pH = 7.2, respectively. The R_2ex of glutamine at pH = 6.1-6.7 was in the 0.097-0.1 s^-1 mM^-1 range. No significant dependence of T₁ on the concentration of glutamine was observed. The dynamic measurement of T₂ -weighted images in vivo showed that the glutamine uptake was primarily observed at the localized part of the tumor CONCLUSION: L-glutamine can be used as a T₂ exchange contrast agent and images of glutamine uptake in vivo can be acquired.

Keywords: cancer imaging; exchange contrast agent; glutamine.