Whole-genome sequence of a reassortant G9P[4] rotavirus A strain from two children in the Czech Republic

Arch Virol. 2020 Jul;165(7):1703-1706. doi: 10.1007/s00705-020-04648-w. Epub 2020 May 13.

Abstract

An unusual reassortant rotavirus A (RVA) strain was isolated during RVA surveillance in two previously hospitalized children in 2018. G/P typing revealed uncommon G9P[4] genotypes, so the strains were further characterized by Illumina next-generation sequencing. Whole-genome typing showed that the two strains had a DS-1-like backbone except for NSP2: G9-P[4]-I2-R2-C2-M2-A2-N1-T2-E2-H2. The two strains shared 99.9-100% nucleotide sequence identity in all genes.

MeSH terms

  • Base Sequence
  • Czech Republic
  • Female
  • Genome, Viral
  • Humans
  • Infant
  • Phylogeny
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / virology*
  • Whole Genome Sequencing