The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa (D. viscosa) and to assess their antimicrobial potency by established in-vitro agar diffusion method. The methanol extract was liquefied and fractioned by using a separatory funnel and organic solvents having different polarities. The agar diffusion technique was used to assess the antimicrobial potency of extracts and pure compounds against 5 g (+and -) microbial strains. Antimicrobial potency results showed that all extracts and isolated pure compounds provided significant antimicrobial potency against the applied microbial strains. The highest activity chloroform extract was analysed by column chromatography (CC) for the isolation of antimicrobial compounds. The structure of the isolated compounds was characterized based on 1D, 2D NMR and MS spectra. In conclusion, purest compounds might be useful as a remedy for infectious diseases.
Keywords: Antimicrobial compounds; Antimicrobial potency; Chromatography; Dodonaea viscosa; MS; NMR; Soxhlet.
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