Soil fungi have been widely studied, but the effects of heavy metal contamination at various levels as well as the abundance and diversity of heavy metal tolerant fungi in the contaminated paddy soils are still unknown. The purpose of this study is to analyze the adaptability of fungi at different levels of heavy metal contamination to identify species that have strong adaptability to heavy metals. In this research, the technology of high-throughput sequencing was applied to study fungal communities in severe level (SL), moderate level (ML), light level (LL), and clean level (CL) for soil samples polluted by heavy metal, as well as to analyze the relations between environmental variables and fungal communities. The spearman analysis showed that 6 dominant fungal phyla and 18 dominant fungal genera were significantly correlated with these environmental variables. The α-diversity indexes of the soil fungal community from SL, ML, and CL were, mostly, drastically higher than the LL samples (p < 0.05). Meanwhile, Ascomycota, the main fungal phylum, was spotted to yield a strong tolerance towards heavy metals, especially in ML. The most dominant genera of tolerant fungi in this area, which are Aspergillus, Penicillium, and Fusarium, could absorb and transport the heavy metals with the help of nutrients under certain heavy metal contamination levels. Therefore, this study indicated that some fungi, which have strong biodegradability on heavy metals, can reduce toxicity of heavy metals and create a proper soil environment to grow food crops. Graphical abstract.
Keywords: Heavy metal contamination; High-throughput sequencing; Paddy fields; Soil fungi.