RuBisCO nano enzyme for mimicking CO2 conversion system in plants

Abstract

In this study, carbon dioxide (CO₂ ) capture and conversion systems based on the combination of biomimetic systems with nano enzymes have been developed. The effectiveness of the developed system has been investigated toward CO₂ conversion. For this aim, nano ribulose bisphosphate carboxylase/oxygenase (RuBisCO) enzyme that plays role in the Calvin cycle in photosynthesis has been synthesized in 93 nm size according to AmiNoAcid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method. Enzymatic activity of synthesized nano RuBisCO enzyme has been spectrophotometrically determined by the formation of 3-phosphoglycerate (3-PGA) at the end of the reaction between CO₂ and d-ribulose-1,5 biphosphate with the catalysis of RuBisCO enzyme at 340 nm. The effect of substrate concentration, pH, temperature, and Mg²⁺ ion concentration on the conversion reaction have investigated comparatively with nano and free RuBisCO enzyme. Besides this, the reusability feature of synthesized nano RuBisCO enzyme in conversion of CO₂ reaction is indicated. When all data were evaluated, it has been seen that the nano RuBisCO enzyme is effective on the conversion of CO₂ into 3-PGA and can be used for CO₂ capture and conversion systems repeatedly without any deformation in its structure.

Keywords: ANADOLUCA; RuBisCO enzyme; enzymatic CO2 conversion.