The analytical determination of polyphosphates in food: A point-to-point comparison between direct ion chromatography and indirect photometry

Marco Iammarino; Naceur Haouet; Aurelia Di Taranto; Giovanna Berardi; Ferdinando Benedetti; Sara Di Bella; Antonio Eugenio Chiaravalle

doi:10.1016/j.foodchem.2020.126937

The analytical determination of polyphosphates in food: A point-to-point comparison between direct ion chromatography and indirect photometry

Food Chem. 2020 Apr 27:325:126937. doi: 10.1016/j.foodchem.2020.126937. Online ahead of print.

Authors

Marco Iammarino¹, Naceur Haouet², Aurelia Di Taranto³, Giovanna Berardi⁴, Ferdinando Benedetti⁵, Sara Di Bella⁶, Antonio Eugenio Chiaravalle⁷

Affiliations

¹ Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: marco.iammarino@tin.it.
² Food Control Laboratory, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia, Italy. Electronic address: mn.haouet@izsum.it.
³ Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: aurelia.ditaranto@izspb.it.
⁴ Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: giovanna.berardi@izspb.it.
⁵ Food Control Laboratory, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia, Italy. Electronic address: f.benedetti@izsum.it.
⁶ Food Control Laboratory, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia, Italy. Electronic address: s.dibella@izsum.it.
⁷ Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: eugenio.chiaravalle@izspb.it.

PMID: 32387985
DOI: 10.1016/j.foodchem.2020.126937

Abstract

Being an indirect approach, the standard method for the determination of polyphosphates (PPs) in food maybe characterized by high variability which can lead to significant errors in the final measurement. In this study, this method was validated and then compared to the ion chromatography-based approach. Method trueness and measurement uncertainty resulted comparable, while the precision of ion chromatography approach was higher. No "false positive" responses were obtained from ion chromatography analysis, while indirect photometry may cause "false positive" results (5% of analyzed samples). Regarding "false negative" results, this possibility maybe verified for all food types tested and for seafood when using indirect photometry and ion chromatography, respectively. The percentages of "false negative" samples were equal to 23.3% for indirect photometry and 10% for ion chromatography. In conclusion, the indirect photometry is a reliable method especially as "screening technique", while ion chromatography maybe used as a confirmatory technique, successfully.

Keywords: Calgon (Hexametaphosphate, sodium salt) (PubChem CID: 24968); Diphosphate; Food safety; Indirect photometry; Ion chromatography; Method comparison; Polyphosphate; Potassium pyrophosphate (PubChem CID: 23740); Sodium triphosphate (PubChem CID: 24455); Triphosphate; Validation.