Myorelaxant action of the Dysphania ambrosioides (L.) Mosyakin & Clemants essential oil and its major constituent α-terpinene in isolated rat trachea

Luís Pereira-de-Morais; Andressa de Alencar Silva; Renata Evaristo Rodrigues da Silva; Daniela Maria do Amaral Ferraz Navarro; Henrique Douglas Melo Coutinho; Irwin Rose Alencar de Menezes; Marta Regina Kerntopf; Francisco Assis Bezerra da Cunha; José Henrique Leal-Cardoso; Roseli Barbosa

doi:10.1016/j.foodchem.2020.126923

Myorelaxant action of the Dysphania ambrosioides (L.) Mosyakin & Clemants essential oil and its major constituent α-terpinene in isolated rat trachea

Food Chem. 2020 May 1:325:126923. doi: 10.1016/j.foodchem.2020.126923. Online ahead of print.

Affiliations

¹ Regional University of Cariri, Pimenta Campus, 63105-010 Crato, CE, Brazil; State University of Ceará, Itapery Campus, 60741-000 Fortaleza, CE, Brazil. Electronic address: luispereira256@gmail.com.
² State University of Ceará, Itapery Campus, 60741-000 Fortaleza, CE, Brazil.
³ Biological Chemistry Department, Postgraduate Program in Biological Chemistry, Brazil; Regional University of Cariri, Pimenta Campus, 63105-010 Crato, CE, Brazil.
⁴ Federal University of Pernambuco, University City, 50670-901 Recife, PE, Brazil.

PMID: 32387952
DOI: 10.1016/j.foodchem.2020.126923

Abstract

This study aimed to investigate the myorelaxant action of the Dysphania ambrosioides essential oil (EODa) and its major constituent α-terpinene on tracheal smooth muscle isolated from rats. In tracheal smooth muscle ex vivo, in organ baths, isometric contractions recordings were done in order to evaluated the effect of EODa (1-1000 μg/mL) and α-terpinene (1-3000 μg/mL) on the following parameters: basal tone, contractions evoked by potassium (KCl 60 mM), acetylcholine (ACh 10 μM) or serotonin (5-HT 10 μM). The EODa and its major constituent α-terpinene, did not statistically alter basal tone; however, they induced myorelaxant effects on top of contractions induced by KCl, ACh and 5-HT. EODa and α-terpinene also inhibited the contractions induced by barium in presence of High [K⁺] (80 mM). The data suggest that the relaxation induced by these agents is caused by the inhibition of L-type VGCC, inhibiting the inward Ca²⁺ current through these channels, but does not exclude the possibility of participation of other mechanisms. Results from this study also suggest the EODa, due to their efficacy on relaxation of the respiratory tract, posses a therapeutic potential as a antispasmodic agent for respiratory tract.

Keywords: Antispasmodic effect; Dysphania ambrosioide; Essential oil; Trachea; α-Terpinene.