A new analytical method for the simultaneous quantification of isoflavones and lignans in 25 green coffee samples by HPLC-MS/MS

Simone Angeloni; Luciano Navarini; Gulzhan Khamitova; Filippo Maggi; Gianni Sagratini; Sauro Vittori; Giovanni Caprioli

doi:10.1016/j.foodchem.2020.126924

A new analytical method for the simultaneous quantification of isoflavones and lignans in 25 green coffee samples by HPLC-MS/MS

Food Chem. 2020 Apr 27:325:126924. doi: 10.1016/j.foodchem.2020.126924. Online ahead of print.

Authors

Simone Angeloni¹, Luciano Navarini², Gulzhan Khamitova¹, Filippo Maggi³, Gianni Sagratini³, Sauro Vittori³, Giovanni Caprioli⁴

Affiliations

¹ School of Pharmacy, University of Camerino, via Sant' Agostino 1, 62032 Camerino, Italy; International Hub for Coffee Research and Innovation, Belforte del Chienti (MC), Italy.
² illycaffè S.p.A., via Flavia 110, Trieste I-34147, Italy.
³ School of Pharmacy, University of Camerino, via Sant' Agostino 1, 62032 Camerino, Italy.
⁴ School of Pharmacy, University of Camerino, via Sant' Agostino 1, 62032 Camerino, Italy. Electronic address: giovanni.caprioli@unicam.it.

PMID: 32387932
DOI: 10.1016/j.foodchem.2020.126924

Abstract

Green coffee, the raw material of roasted coffee and coffee beverages, is one of the most widely traded commodities worldwide. There is a dearth of studies on its content in phytoestrogens such as isoflavones and lignans. Previously, we developed an efficient method for the simultaneous quantification of 6 isoflavones (daidzin, genistin, daidzein, genistein, formononetin and biochanin A) and 3 lignans (secoisolariciresinol, matairesinol and lariciresinol) in green coffee by using HPLC-MS/MS. Several extraction processes were evaluated and the best performing, base hydrolysis followed by enzymatic digestion, was validated and used to analyse 25 different coffee samples, 1 Coffea canephora and 24 Coffea arabica, from different countries. Lignans (total content: 286.5-8131.8 µg kg^-1) were found in higher concentration than isoflavones (total content: 3.4-300.0 µg kg^-1) and the most abundant were secoisolariciresinol (172.6-5714.1 µg kg^-1) and lariciresinol (113.9-2417.7 µg kg^-1). Notably, the Ethiopian coffee samples contained the highest levels of these compounds.

Keywords: Biochanin A (PubChem CID 5280373); Daidzein (PubChem CID 5281708); Daidzin (PubChem CID 107971); Extraction process; Formononetin (PubChem CID 5280378); Genistein (PubChem CID 5280961); Genistin (PubChem CID 5281377); Green coffee; HPLC-MS/MS; Isoflavone; Lariciresinol (PubChem CID 332427); Lignan; Matairesinol (PubChem CID 119205); Phytoestrogen; Secoisolariciresinol (PubChem CID 65373).