Antibacterial mechanism of the polysaccharide produced by Chaetomium globosum CGMCC 6882 against Staphylococcus aureus

Int J Biol Macromol. 2020 Sep 15:159:231-235. doi: 10.1016/j.ijbiomac.2020.04.269. Epub 2020 May 5.

Abstract

GCP, a polysaccharide produced by endophytic fungus Chaetomium globosum CGMCC 6882, was found to be antibacterial against Staphylococcus aureus via disrupting cell permeability. However, the antibacterial mechanism of GCP has not been studied before. In present work, results showed that GCP could retard the growth of S. aureus by inducing the depolarization of cell membrane, decreasing the activity of Ca2+-Mg2+-ATPase on cell membrane, and increasing the accumulation of calcium ions in cytoplasm. Moreover, we found that GCP could also inhibit the synthesis of whole cell proteins of S. aureus. Overall, this study proved that the antibacterial mechanism of GCP could be diversified and more studies are needed in the investigation of the antibacterial mechanisms of various polysaccharides.

Keywords: Antibacterial mechanism; Chaetomium globosum CGMCC 6882; Polysaccharide.

MeSH terms

  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Calcium / metabolism
  • Chaetomium / chemistry*
  • Cytoplasm
  • DNA Replication / drug effects
  • Enzyme Activation
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / pharmacology*
  • Membrane Potentials / drug effects
  • Microbial Sensitivity Tests
  • Staphylococcus aureus / drug effects*

Substances

  • Anti-Bacterial Agents
  • Fungal Polysaccharides
  • Calcium

Supplementary concepts

  • Chaetomium globosum