Pectin-microfibrillated cellulose microgel: Effects on survival of lactic acid bacteria in a simulated gastrointestinal tract

Bingyan Chen; Xiaozi Lin; Xiaojie Lin; Weixin Li; Baodong Zheng; Zhigang He

doi:10.1016/j.ijbiomac.2020.04.161

Pectin-microfibrillated cellulose microgel: Effects on survival of lactic acid bacteria in a simulated gastrointestinal tract

Int J Biol Macromol. 2020 May 6:158:826-836. doi: 10.1016/j.ijbiomac.2020.04.161. Online ahead of print.

Authors

Bingyan Chen¹, Xiaozi Lin², Xiaojie Lin², Weixin Li², Baodong Zheng³, Zhigang He⁴

Affiliations

¹ Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350002, China; Fujian Province Key Laboratory of Agricultural Products (Food) Processing Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350002, China; Fujian Province Key Laboratory of Agricultural Products (Food) Processing Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350002, China.
³ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
⁴ Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350002, China; Fujian Province Key Laboratory of Agricultural Products (Food) Processing Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350002, China. Electronic address: njgzx@163.com.

PMID: 32387360
DOI: 10.1016/j.ijbiomac.2020.04.161

Abstract

Using high pressure microfluidization, we prepared micro-fibrillated soybean cellulose (MFSC) and analyzed its morphology and structure. MFSC was then incorporated into low-methoxyl pectin (PC) to coat lactic acid bacteria (LAB) by ionotropic gelation, and the effects of PC-MFSC microgel on LAB survival in a simulated gastrointestinal tract were investigated. Particle size analysis showed that the MFSC particle size decreased significantly with increasing jet pressure. Transmission electron microscopy analysis indicated that many cellulosic microfibers appeared at 150 MPa. Infrared spectroscopy and X-ray diffraction analysis revealed that the crystal structure changed from β-cellulose I type to II type with increasing jet pressure, but excessive pressure (200 MPa) damaged the crystalline structure of MFSC. Scanning microscopy indicated that cellulosic microfibers not only promoted a compact pectin gel morphology but also adhered to and coated the LAB in the pectin gel. MFSC-150 stabilized the pectin gel network, preventing the weakening of the gel under low pH conditions. Compared with other PC-MFSCs, PC-MFSC-150 microgel significantly decreased LAB susceptibility to gastrointestinal juice and increased the viability of LAB.

Keywords: Cellulosic microfibers; Encapsulation; Pectin; Probiotics; Simulated gastrointestinal tract.