Objectives: To investigate the effect of propofol on invasion and migration of human colon cancer cell line SW480 and the JAK2/STAT3 pathway.
Methods: Human colon cancer cell line SW480 was divided into a control group, 3 propofol treatment groups (with 2, 4 and 8 μg/mL propofol treatment, respectively), and a propofol+colivelin group. Lactate dehydrogenase (LDH) activity and cell proliferation was evaluated by detection kit and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, respectively. Cell migration and invasion were detected by Transwell assay. Western blotting was performed to detect the protein expression levels of JAK2, p-JAK 2, STAT3 and p-STAT3 in colon cancer cell line SW480.
Results: Compared with the control group, the LDH activity was significantly increased in the propofol treatment groups (P<0.05). The cell proliferation, migration, invasion as well as p-JAK2 and p-STAT3 in colon cancer cell line SW480 in the propofol treatment groups were significantly decreased (allP<0.05). Compared with the propofol treatment groups, the p-STAT3 level, proliferation, migration and invasion in the colon cancer cell line SW480 were significantly increased in the propofol+colivelin group (allP<0.05).
Conclusions: Propofol can inhibit the proliferation, migration and invasion of human colon cancer cells by inhibiting JAK2/STAT3 signaling pathway.
目的: 探讨丙泊酚对人结肠癌细胞株SW480侵袭、迁移的作用及对Janus激酶2/信号转导与转录激活子3(Janus kinase 2/signal transduction and transcriptional activator 3，JAK2/STAT3)信号通路的调控。方法: 人结肠癌细胞株SW480分为空白对照组、丙泊酚处理组(又分为2，4，8 μg/mL丙泊酚处理组)和丙泊酚+colivelin组，用乳酸脱氢酶(lactate dehydrogenase, LDH)活性检测试剂盒检测LDH活性，用噻唑蓝(methyl thiazolyl tetrazolium，MTT)法检测细胞增殖，用Transwell实验检测细胞迁移和侵袭，用蛋白质印迹法检测细胞中JAK2，磷酸化JAK2(p-JAK2)，STAT3和磷酸化STAT3(p-STAT3)的蛋白表达量。结果: 与空白对照组相比，丙泊酚处理组细胞株SW480的LDH活性显著升高(P<0.05)，细胞增殖、迁移和侵袭能力以及p-JAK2和p-STAT3的表达均显著下降(均P<0.05)。与丙泊酚处理组相比，丙泊酚+colivelin组SW480细胞p-STAT3的表达水平、细胞增殖、迁移和侵袭能力均显著升高(均P<0.05)。结论: 丙泊酚能够通过抑制JAK2/STAT3信号通路而抑制人结肠癌细胞的增殖和转移。.
Keywords: Janus kinase 2/signal transduction and transcriptional activator 3; colon cancer; invasion; migration; propofol.