An accumulation of two populations of dendritic cells in skin-draining lymph nodes in response to the expression of thymic stromal lymphopoietin in the skin

Miyuki Omori-Miyake; Hiroshi Watarai; Kayoko Sato; Steven F Ziegler; Junji Yagi

doi:10.1016/j.cellimm.2020.104116

An accumulation of two populations of dendritic cells in skin-draining lymph nodes in response to the expression of thymic stromal lymphopoietin in the skin

Cell Immunol. 2020 Jul:353:104116. doi: 10.1016/j.cellimm.2020.104116. Epub 2020 Apr 29.

Authors

Miyuki Omori-Miyake¹, Hiroshi Watarai², Kayoko Sato³, Steven F Ziegler⁴, Junji Yagi⁵

Affiliations

¹ Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan. Electronic address: momorim@m.ehime-u.ac.jp.
² Division of Stem Cell Cellomics, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
³ Department of Cardiology, Tokyo Women's Medical University, Tokyo, Japan.
⁴ Immunology Program, Benaroya Research Institute, Seattle, WA, USA.
⁵ Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.

PMID: 32380184
DOI: 10.1016/j.cellimm.2020.104116

Abstract

Thymic stromal lymphopoietin (TSLP) acts on dendritic cells (DCs), which prime helper T (Th) cells to become type 2 cytokine producing cells. Recently, a different set of populations of TSLP-responsive DCs has been discovered. Here, we identified two populations of CD103^loEpCAM^hi migratory DCs (fraction I and fraction II) that accumulated in skin-draining lymph nodes in response to TSLP expressed in the mouse skin. Fraction I DCs with CD11b⁺PDL2^hi expression primed naïve Th cells to differentiate into cells secreting IFN-γ, IL-17A and IL-22, while fraction II DCs with CD11b^loPDL2⁺ expression primed naïve Th cells to differentiate into cells secreting IL-4, IL-5, IL-9, IL-13 and IL-10. Fraction I DCs migrated from the skin via IL-4Rα signaling pathway, whereas fraction II DCs migrated partially via TSLPR signaling pathway. All suggest that at least two populations of CD103^loEpCAM^hi DCs with distinct functions and pathways could migrate in response to TSLP expression in the skin.

Keywords: Allergic dermatitis; Dendritic cell; TSLP-responsive populations of dendritic cell; Thymic stromal lymphopoietin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD / immunology
Antigens, CD / metabolism
Cytokines / metabolism*
Cytokines / physiology
Dendritic Cells / metabolism*
Dendritic Cells / physiology
Female
Integrin alpha Chains / immunology
Integrin alpha Chains / metabolism
Interleukin-17 / metabolism
Lymph Nodes / metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Transgenic
Skin / metabolism
T-Lymphocytes, Helper-Inducer / metabolism
Th2 Cells / metabolism
Thymic Stromal Lymphopoietin

Substances

Antigens, CD
Cytokines
Integrin alpha Chains
Interleukin-17
alpha E integrins
Thymic Stromal Lymphopoietin
TSLP protein, mouse