Knockdown of CDCA8 inhibits the proliferation and enhances the apoptosis of bladder cancer cells

Xin Gao; Xiaohong Wen; Haowei He; Linlin Zheng; Yibo Yang; Jinlian Yang; Haifang Liu; Xiguo Zhou; Changshun Yang; Yinyi Chen; Mei Chen; Shufang Zhang

doi:10.7717/peerj.9078

Knockdown of CDCA8 inhibits the proliferation and enhances the apoptosis of bladder cancer cells

PeerJ. 2020 Apr 28:8:e9078. doi: 10.7717/peerj.9078. eCollection 2020.

Authors

Xin Gao^#^{1

2}, Xiaohong Wen^#¹, Haowei He¹, Linlin Zheng¹, Yibo Yang², Jinlian Yang², Haifang Liu¹, Xiguo Zhou², Changshun Yang², Yinyi Chen^{1

3}, Mei Chen¹, Shufang Zhang¹

Affiliations

¹ Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, Hainan, China.
² Clinical Laboratory, The First People's Hospital of Huaihua of University of South China, Huaihua, China.
³ Clinical Laboratory, The First Affiliated Hospital of University of South China, Hengyang, China.

^# Contributed equally.

Abstract

Bladder cancer is a tumour of the urinary system with high mortality, and there is also a great lack of therapeutic targets in the clinic. Cell division cycle associated 8 (CDCA8), an important component of the vertebrate chromosomal passenger complex, is highly expressed in various tumours and promotes tumour development. However, the role of CDCA8 in bladder cancer is not fully understood. This study aimed to reveal the function of CDCA8 in bladder cancer by determining the relationship between CDCA8 expression and proliferation, metastasis and apoptosis of bladder cancer cells. Firstly, we studied the mRNA expression of CDCA8 through the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) databases and analysed the correlation between CDCA8 expression and prognosis of patients with bladder cancer. We also verified CDCA8 expression in bladder cancer tissues by immunohistochemistry. In addition, CDCA8 expression was inhibited in bladder cancer T24 and 5637 cells, and the effects of CDCA8 on the proliferation, migration and invasion of bladder cancer cell lines were investigated using cell counting kit-8, colony formation, cell cycle, apoptosis, wound healing and Transwell invasion assays. Results showed that CDCA8 was highly expressed in bladder cancer compared with normal tissues, and the high CDCA8 expression was significantly correlated with the poor prognosis of patients. Inhibiting CDCA8 expression inhibited the proliferation, migration and invasion of T24 and 5637 cells and induced the apoptosis of bladder cancer cells. CDCA8 was involved in the regulation of the growth cycle of bladder cancer cells. Bioinformatics-based mechanism analysis revealed that high CDCA8 expression may affect the cell cycle and P53 signalling pathways. In conclusion, our results suggest that CDCA8 is highly expressed in bladder cancer and can promote tumour development. Hence, CDCA8 may serve as an effective therapeutic target for treatment of bladder cancer.

Keywords: Bladder cancer; Cell division cycle associated 8; Prognosis; Proliferation; Therapeutic target.

Associated data

figshare/10.6084/m9.figshare.10311638.v1

Grants and funding

This study was supported by the Hainan Provincial Natural Science Foundation of China (Grant No. 819QN387, 2017CXTD010, 20168312), the National Science Foundation of China (Grant No. 81760465), and the Finance science and technology project of hainan province (Grant No. ZDYF2019163 and ZDKJ2017007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.