Analysis of microRNA expression in peripheral blood monocytes of three Traditional Chinese Medicine (TCM) syndrome types in psoriasis patients

Yue Lu; Yao Qi; Yuhong Yan; Danni Yao; Hao Deng; Jingwen Deng; Shuyan Ye; Haiming Chen; Qubo Chen; Hengjun Gao; Ling Han; Chuanjian Lu

doi:10.1186/s13020-020-00308-y

Analysis of microRNA expression in peripheral blood monocytes of three Traditional Chinese Medicine (TCM) syndrome types in psoriasis patients

Chin Med. 2020 May 1:15:39. doi: 10.1186/s13020-020-00308-y. eCollection 2020.

Authors

Yue Lu^{1

2

3}, Yao Qi^{4

5}, Yuhong Yan^{1

2

3}, Danni Yao^{1

2

3}, Hao Deng^{1

2

3}, Jingwen Deng^{1

2

3}, Shuyan Ye^{1

2

3}, Haiming Chen^{1

2

3}, Qubo Chen^{1

2

3}, Hengjun Gao^{4

5}, Ling Han^{1

2

3

6}, Chuanjian Lu^{1

2

3

6}

Affiliations

¹ 1Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510006 Guangdong People's Republic of China.
² Guangdong Academy of Traditional Chinese Medicine, Guangzhou, 510006 Guangdong People's Republic of China.
³ 3The Second Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, 510006 Guangdong People's Republic of China.
⁴ Shanghai Molecular Medicine Engineering Technology Research Center, Shanghai, 201203 China.
⁵ Shanghai National Engineering Research Center of Biochip, Shanghai, 201203 China.
⁶ 4Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou, 510006 Guangdong People's Republic of China.

Abstract

Background: To analyze the expression of miRNA (microRNA) in peripheral blood mononuclear cells in patients with Psoriasis vulgaris with different TCM syndromes by miRNA chip. It further revealed the micromaterial basis of different syndrome types of psoriasis at the miRNA level.

Methods: Peripheral blood monocytes were collected and prepared from 30 patients with Psoriasis vulgaris (including 9 patients of blood heat syndrome, 8 patients of blood stasis syndrome, and 13 patients of blood dry syndrome) and 9 healthy controls. The miRNA expression profile of peripheral blood monocytes was detected by Agilent Hum miRNA chip.

Results: Compared to the healthy control group, 156 upregulated and 242 downregulated miRNAs were detected in all psoriasis patients. Compared to the healthy control group, 40 miRNAs were upregulated and 44 were downregulated in the blood heat syndrome group. Furthermore, there were 49 upregulated miRNAs and 44 downregulated miRNAs in the dry syndrome group as compared to the healthy control group. Also, 67 miRNAs were upregulated and 154 miRNAs were downregulated in the blood stasis syndrome group as compared to the healthy control group.

Conclusions: There are common different miRNAs and pathways, as well as specific miRNAs between the psoriasis and the healthy control groups.Trial registration ChiCTR-TRC-14005185 registered on August 8, 2014.

Keywords: Psoriasis; TCM syndrome type; miRNA chip; miRNA expression.