Anti-flavivirus activity of polyoxometalate

Yue Qi; Lin Han; Yanfei Qi; Xia Jin; Bo Zhang; Junqi Niu; Jin Zhong; Yongfen Xu

doi:10.1016/j.antiviral.2020.104813

Anti-flavivirus activity of polyoxometalate

Antiviral Res. 2020 Jul:179:104813. doi: 10.1016/j.antiviral.2020.104813. Epub 2020 May 4.

Authors

Yue Qi¹, Lin Han², Yanfei Qi³, Xia Jin⁴, Bo Zhang⁵, Junqi Niu⁶, Jin Zhong², Yongfen Xu⁷

Affiliations

¹ Department of Hepatology, First Hospital, Jilin University, Changchun, Jilin, 130021, China. Electronic address: qq9377@sina.com.
² CAS Key Laboratory of Molecular Virology and Immunology, Unit of Viral Hepatitis, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China; Shanghai Tech University, Shanghai, 201210, China.
³ Department of Hepatology, First Hospital, Jilin University, Changchun, Jilin, 130021, China; School of Public Health, Jilin University, Changchun, Jilin, 130021, China.
⁴ CAS Key Laboratory of Molecular Virology and Immunology, Unit of Viral Hepatitis, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China.
⁵ Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan, 430071, China.
⁶ Department of Hepatology, First Hospital, Jilin University, Changchun, Jilin, 130021, China.
⁷ CAS Key Laboratory of Molecular Virology and Immunology, Unit of Viral Hepatitis, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address: xuyf@ips.ac.cn.

PMID: 32376449
DOI: 10.1016/j.antiviral.2020.104813

Abstract

Viruses in the Flaviviridae family such as Zika virus (ZIKV), dengue virus (DENV), and Japanese encephalitis virus (JEV) are major public health concerns. The development of antiviral agents against these viruses is urgently needed. We have previously discovered that the Keggin structured polyoxometalate POM-12 has potent inhibitory activity against hepatitis C virus, another member of the Flaviviridae family. In this study, we tested its antiviral activity of DENV, JEV and ZIKV, and found that POM-12 dramatically inhibited their infection with IC50 value of 1.16 μM, 1.9 μM and 0.64 μM, respectively. Mechanistic studies indicated that POM-12 directly disrupted the integrity of these virions. Moreover, POM-12 also targeted the post-entry steps of viral replication of JEV, but having no similar activities on ZIKV and DENV. The differential actions of POM-12 on these viruses suggest that surface topology and charge of virion may have influence on its drug effect, and thus POM-12 may be modified to more efficiently inhibit these and other similar viruses.

Keywords: Anti-Viral; DENV; JEV; Polyoxometalate; Virucidal; ZIKV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anions / pharmacology*
Antiviral Agents / pharmacology*
Chlorocebus aethiops
Dengue Virus / drug effects
Drug Discovery
Encephalitis Virus, Japanese / drug effects
Flavivirus / classification
Flavivirus / drug effects*
Flavivirus / physiology
Inhibitory Concentration 50
Polyelectrolytes / pharmacology*
Vero Cells
Virus Replication / drug effects*
Zika Virus / drug effects

Substances

Anions
Antiviral Agents
Polyelectrolytes
polyoxometalate