Comprehensive proteomics and functional annotation of mouse brown adipose tissue

Jing Li; Juan Li; Wei-Gang Zhao; Hai-Dan Sun; Zheng-Guang Guo; Xiao-Yan Liu; Xiao-Yue Tang; Zhu-Fang She; Tao Yuan; Shuai-Nan Liu; Quan Liu; Yong Fu; Wei Sun

doi:10.1371/journal.pone.0232084

Comprehensive proteomics and functional annotation of mouse brown adipose tissue

PLoS One. 2020 May 6;15(5):e0232084. doi: 10.1371/journal.pone.0232084. eCollection 2020.

Authors

Jing Li¹, Juan Li², Wei-Gang Zhao², Hai-Dan Sun¹, Zheng-Guang Guo¹, Xiao-Yan Liu¹, Xiao-Yue Tang¹, Zhu-Fang She³, Tao Yuan², Shuai-Nan Liu³, Quan Liu³, Yong Fu², Wei Sun¹

Affiliations

¹ Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing, China.
² Key Laboratory of Endocrinology of Ministry of Health, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
³ State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Diabetes Research Center of Chinese Academy of Medical Sciences, Beijing, China.

Abstract

Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue, Brown / chemistry
Adipose Tissue, Brown / metabolism*
Animals
Chromatography, High Pressure Liquid
Chromatography, Liquid
Databases, Protein
Female
Mice
Mice, Inbred C57BL
Molecular Sequence Annotation* / methods
Proteome / analysis
Proteome / metabolism*
Proteomics* / methods
Tandem Mass Spectrometry

Substances

Proteome

Grants and funding

This work was supported by National Basic Research Program of China (No. 2013CB530805, 2014CBA02005), National Key Research and Development Program of China (No. 2016 YFC 1306300,2018YFC0910202), Key Basic Research Program of the Ministry of Science and Technology of China (No. 2013FY114100), National Natural Science Foundation of China (No. 30970650, 31200614, 31400669, 81371515, 81170665, 81560121), Beijing Natural Science Foundation (No. 7173264, 7172076), Beijing cooperative construction project (No.110651103), Beijing Science Program for the Top Young (No.2015000021223TD04), Beijing Normal University (No.11100704), Peking Union Medical College Hospital (No.2016-2.27), CAMS Innovation Fund for Medical Sciences (2017-I2M-1-009) and Biologic Medicine Information Center of China, National Scientific Data Sharing Platform for Population and Health.