Cajanin Stilbene Acid Inhibited Vancomycin-Resistant Enterococcus by Inhibiting Phosphotransferase System

Shengnan Tan; Xin Hua; Zheyong Xue; Jianzhang Ma

doi:10.3389/fphar.2020.00473

Cajanin Stilbene Acid Inhibited Vancomycin-Resistant Enterococcus by Inhibiting Phosphotransferase System

Front Pharmacol. 2020 Apr 15:11:473. doi: 10.3389/fphar.2020.00473. eCollection 2020.

Authors

Shengnan Tan^{1

2}, Xin Hua^{1

3}, Zheyong Xue^{1

4

3}, Jianzhang Ma²

Affiliations

¹ Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, China.
² College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China.
³ College of Life Science, Northeast Forestry University, Harbin, China.
⁴ College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, China.

Abstract

Antimicrobial resistance has become a serious threat to human and animal health, and vancomycin-resistant Enterococcus has become an important nosocomial infection pathogen, causing thousands of deaths each year. In this study, after screening a variety of natural products, we found that cajanin stilbene acid (CSA) had significant inhibitory effect on sensitive and vancomycin-resistant Enterococcus (VRE) in vitro. And we also confirmed that CSA had significant anti-VRE infection ability in vivo. Subsequently, we studied the antibacterial mechanism of CSA through proteomics experiments, and the results showed that CSA killed Enterococcus by inhibiting the phosphotransferase system of Enterococcus, thus hinders the normal growth and metabolic functions of bacteria. The results of this study provided evidence for the in-depth study on the mechanism of the antibacterial action of CSA and also provided a candidate for the development of anti-VRE drugs.

Keywords: cajanin stilbene acid (CSA); inhibition mechanism; phosphotransferase system (PTS); proteomics; vancomycin-resistant Enterococcus (VRE).