Generation of RUNX1-null reporter human embryonic stem cell line GIBHe008-A

Hanif Ullah; Hao You; Zahir Shah; Chenyu Fan; Baoyun Zhang; Hao Liu; Jianguang Zhang; Nasir Abbas; Elena S Filonenko; Igor M Samokhvalov

doi:10.1016/j.scr.2020.101800

Generation of RUNX1-null reporter human embryonic stem cell line GIBHe008-A

Stem Cell Res. 2020 May:45:101800. doi: 10.1016/j.scr.2020.101800. Epub 2020 Apr 21.

Authors

Affiliations

¹ Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
² Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510630, China.
³ Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Institute of Public Health, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, 510530, China.
⁴ Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: igor@gibh.ac.cn.

PMID: 32371361
DOI: 10.1016/j.scr.2020.101800

Abstract

RUNX1/AML1/CBFA2 (runt-related transcription factor 1/acute myeloid leukemia 1 protein/core-binding factor subunit alpha-2), is a transcription factor that plays a critical role in the development of normal hematopoiesis. RUNX1 is also essential for the development of immune cells and sensory neurons. Chromosomal translocations involving the gene have been associated with several types of leukemia. To investigate the role of RUNX1 in human hematopoietic development we generated RUNX1-null human embryonic stem cell reporter line GIBHe008-A by TALEN mediated homologous recombination. This cell line GIBHe008-A was subjected to detailed characterization by standard assays for human pluripotent stem cells. It provides an ideal model to study the role of RUNX1 in the hESC-derived developmental models.

Keywords: Gene targeting; Human embryonic stem cells (hESCs); In vitro differentiation; RUNX1; TALEN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Core Binding Factor Alpha 2 Subunit* / genetics
Embryonic Stem Cells
Hematopoiesis
Human Embryonic Stem Cells*
Humans

Substances

Core Binding Factor Alpha 2 Subunit
RUNX1 protein, human