Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation

Methods Mol Biol. 2020:2142:81-92. doi: 10.1007/978-1-0716-0581-3_7.

Abstract

Inflammation is part of a defense reaction of live tissues that is triggered by pathogens, chemical reagents, trauma, and radiation. Understanding the inflammatory process triggered by Zika virus (ZIKV) is important to better understand the pathogen-host interaction. The evaluation of this process can be done using tools such as enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription PCR (RT-qPCR). Both techniques have been an indispensable tool not just for immunologists but for all interested in understanding the inflammatory process.

Keywords: Antigens; Chemokines; Cytokines; ELISA; Inflammation; RT-qPCR; ZIKA virus infection.

MeSH terms

  • Animals
  • Blood-Testis Barrier / immunology
  • Blood-Testis Barrier / metabolism
  • Blood-Testis Barrier / virology
  • Cell Death
  • Enzyme-Linked Immunosorbent Assay / methods
  • Host-Pathogen Interactions / genetics
  • Host-Pathogen Interactions / immunology
  • Humans
  • Inflammation / diagnosis*
  • Inflammation / genetics
  • Inflammation / immunology
  • Inflammation / virology
  • Male
  • Mice
  • Orchitis / diagnosis
  • Orchitis / genetics
  • Orchitis / immunology
  • Orchitis / virology
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Testis / pathology
  • Testis / physiology
  • Zika Virus / immunology
  • Zika Virus / pathogenicity
  • Zika Virus / physiology*
  • Zika Virus Infection / complications
  • Zika Virus Infection / genetics
  • Zika Virus Infection / immunology
  • Zika Virus Infection / metabolism