Evaluating Zika Virus Pathogenesis in Immunocompromised Mice

Methods Mol Biol. 2020:2142:23-40. doi: 10.1007/978-1-0716-0581-3_3.

Abstract

Mouse models of Zika virus (ZIKV) infection, which recapitulate certain aspects of ZIKV infection in humans, have demonstrated their utility for studying ZIKV pathogenesis and for the initial screening of antivirals, experimental therapeutics, and candidate vaccines for protective efficacy against ZIKV infection. In this chapter we outline the available mouse models and describe the evaluation of ZIKV pathogenesis in an immunocompromised mouse model of ZIKV infection.

Keywords: Animal model; Flavivirus; Interferon; Mouse; Pathogenesis; Zika virus.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Female
  • Host-Pathogen Interactions / immunology
  • Humans
  • Immunocompromised Host*
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Receptor, Interferon alpha-beta / deficiency
  • Receptor, Interferon alpha-beta / genetics
  • Viral Plaque Assay
  • Virology / methods
  • Zika Virus / immunology
  • Zika Virus / isolation & purification
  • Zika Virus / pathogenicity*
  • Zika Virus Infection / immunology
  • Zika Virus Infection / pathology*
  • Zika Virus Infection / virology

Substances

  • Ifnar1 protein, mouse
  • Receptor, Interferon alpha-beta