ABHD12 Knockdown Suppresses Breast Cancer Cell Proliferation, Migration and Invasion

Anticancer Res. 2020 May;40(5):2601-2611. doi: 10.21873/anticanres.14231.

Abstract

Background/aim: Alpha/beta-hydrolase domain containing 12 (ABHD12) is a serine hydrolase that regulates immunological and neurological mechanisms. This study aimed to elucidate the oncogenic effect of ABHD12 on human breast cancer.

Materials and methods: ABHD12 expression was confirmed in breast cancer tissues and breast cancer cell lines by immunohistochemistry and quantitative RT-PCR. To determine the role of ABHD12, ABHD12 siRNA-suppressed breast cancer cells (MCF7 and MDA-MB-231 cells) were investigated for cell proliferation, migration, and invasion capabilities using MTT assays, EdU assays, colony formation assays, and Boyden chamber assays.

Results: Immunohistochemical staining showed a higher ABHD12 expression in breast cancer tissues than in normal tissues. Additionally, ABHD12 knockdown was found to inhibit cell growth, proliferation, migration, and invasion in breast cancer cells.

Conclusion: ABHD12 plays a crucial role in cell proliferation, migration, and invasion of breast cancer cells.

Keywords: ABHD12; breast cancer; invasion; migration; proliferation.

MeSH terms

  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Movement* / genetics
  • Cell Proliferation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques*
  • Humans
  • Monoacylglycerol Lipases / genetics
  • Monoacylglycerol Lipases / metabolism*
  • Neoplasm Invasiveness
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / metabolism
  • Receptors, Cannabinoid / metabolism
  • Up-Regulation / genetics

Substances

  • RNA, Messenger
  • RNA, Small Interfering
  • Receptors, Cannabinoid
  • ABHD12 protein, human
  • Monoacylglycerol Lipases