Anti-inflammatory and antipyretic properties of Kang 601 heji, a traditional Chinese oral liquid dosage form

Raphael Nammahime Alolga; Sarah Wambui Amadi; Vitus Onoja; Assogba Gabin Assanhou; Moses Muyaba; Said Abasse Kassim

doi:10.1016/j.apjtb.2015.08.001

Anti-inflammatory and antipyretic properties of Kang 601 heji, a traditional Chinese oral liquid dosage form

Asian Pac J Trop Biomed. 2015 Nov;5(11):921-927. doi: 10.1016/j.apjtb.2015.08.001. Epub 2015 Aug 28.

Authors

Raphael Nammahime Alolga¹, Sarah Wambui Amadi², Vitus Onoja³, Assogba Gabin Assanhou³, Moses Muyaba⁴, Said Abasse Kassim³

Affiliations

¹ State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, China.
² Department of Pharmacology, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, China.
³ Joint Key Laboratory for Drug Development, Department of Pharmaceutics, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing, China.
⁴ Department of Medicinal Chemistry, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, China.

Abstract

Objective: To evaluate the scientific basis for the use of Kang 601 heji (K-601) as an anti-inflammatory and antipyretic agent using appropriate animal models.

Methods: Carrageenan-induced rat paw and xylene-induced ear oedemas were models used to investigate anti-inflammatory actions of K-601. Lipopolysaccharide-induced pyrexia model was used to evaluate antipyretic activity in Wistar rats. The anti-inflammatory and antipyretic mechanisms were evaluated by detecting prostaglandins E₂, nitric oxide, interleukin-1β and tumour necrosis factor-α levels using appropriate reagents and ELISA kits.

Results: The results revealed that K-601 reduced the level of inflammations in both anti-inflammatory models in a dose-dependent manner. The same was true for the antipyretic model. The possible mechanisms of actions were through the inhibition of prostaglandins E₂, interleukin-1β, tumour necrosis factor-α and nitric oxide.

Conclusions: K-601 has proven anti-inflammatory and antipyretic actions. The findings provide a scientific basis for the use of K-601 as anti-inflammatory and antipyretic agent in traditional Chinese medicinal practice.

Keywords: Anti-inflammatory; Antipyretic; Kang 601 heji; Mechanisms; Model.