Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase

Nozomu Tsuruoka; Silvia Sato Soto; Awatef Ben Tahar; Abdelkader Zebda; Seiya Tsujimura

doi:10.1016/j.colsurfb.2020.111065

Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase

Colloids Surf B Biointerfaces. 2020 Apr 21:192:111065. doi: 10.1016/j.colsurfb.2020.111065. Online ahead of print.

Authors

Nozomu Tsuruoka¹, Silvia Sato Soto¹, Awatef Ben Tahar², Abdelkader Zebda², Seiya Tsujimura³

Affiliations

¹ Division of Material Science, Faculty of Pure and Applied Science, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-5358, Japan.
² Université Grenoble Alpes, TIMC-IMAG/CNRS/INSERM, UMR 5525, F-38000, Grenoble, France.
³ Division of Material Science, Faculty of Pure and Applied Science, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-5358, Japan. Electronic address: seiya@ims.tsukuba.ac.jp.

PMID: 32361503
DOI: 10.1016/j.colsurfb.2020.111065

Abstract

Electrochemically polymerized phenothiazines (thionine, methylene green, methylene blue, and toluidine blue) on carbon electrodes were investigated as electron transfer mediators of glucose oxidation by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) for biosensor and biofuel cell applications. Among the tested polyphenothiazines grafted on a glassy carbon electrode, clear redox-mediating activity was observed for poly(methylene green), and the catalytic oxidation current depended on the concentrations of glucose and enzymes and the amount of polymer deposited on the electrode surface. The poly(methylene green)-grafted porous carbon electrodes showed 3 mA cm^-2 of glucose oxidation current catalyzed by FAD-GDH.

Keywords: Bioelectrocatalysis; Electropolymerization; FAD glucose dehydrogenase; Phenothiazine.