Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9

Chun-Lin Liu; Ching-Ying Huang; Hung-Chih Chen; Huai-En Lu; Patrick C H Hsieh; Jia-Jung Lee

doi:10.1016/j.scr.2020.101784

Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9

Stem Cell Res. 2020 May:45:101784. doi: 10.1016/j.scr.2020.101784. Epub 2020 Apr 20.

Authors

Chun-Lin Liu¹, Ching-Ying Huang¹, Hung-Chih Chen², Huai-En Lu³, Patrick C H Hsieh¹, Jia-Jung Lee⁴

Affiliations

¹ Human Disease iPSC Service Consortium, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
² Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
³ Food Industry Research and Development Institute, Hsinchu, Taiwan.
⁴ Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Faculty of Renal Care, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: jjlee7351@gmail.com.

PMID: 32361310
DOI: 10.1016/j.scr.2020.101784

Abstract

We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripotency, and differentiation capacity into three germ layers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Differentiation
Clustered Regularly Interspaced Short Palindromic Repeats
Humans
Induced Pluripotent Stem Cells*
Kidney