Characterization of a specific odorant receptor for linalool in the Chinese citrus fly Bactrocera minax (Diptera: Tephritidae)

Yipeng Liu; Zhongyi Cui; Pinfa Si; Yang Liu; Qiong Zhou; Guirong Wang

doi:10.1016/j.ibmb.2020.103389

Characterization of a specific odorant receptor for linalool in the Chinese citrus fly Bactrocera minax (Diptera: Tephritidae)

Insect Biochem Mol Biol. 2020 Jul:122:103389. doi: 10.1016/j.ibmb.2020.103389. Epub 2020 Apr 28.

Authors

Yipeng Liu¹, Zhongyi Cui², Pinfa Si², Yang Liu³, Qiong Zhou⁴, Guirong Wang⁵

Affiliations

¹ College of Life Sciences, Hunan Normal University, Changsha, 410081, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
² College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
³ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
⁴ College of Life Sciences, Hunan Normal University, Changsha, 410081, China. Electronic address: zhoujoan@hunnu.edu.cn.
⁵ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. Electronic address: wangguirong@caas.cn.

PMID: 32360457
DOI: 10.1016/j.ibmb.2020.103389

Abstract

Insect sensing of odorants plays important roles in various behaviors, including host location, mate attraction, and oviposition site selection. The odorant receptor (OR) is a key protein in insect environmental odor recognition. Most Diptera studies of ORs have focused on Drosophila and mosquitos, so there little known about ORs in the agricultural pest insects Tephritidae. To understand the olfactory recognition mechanism of Bactrocera minax, we sequenced and analyzed 12 B. minax transcriptomes to identify a total of 59 OR genes. Semi-quantitative reverse transcription PCR (RT-PCR) showed that several BminORs were highly expressed in antennae. Available with a complete open reading frame and expressed in the antennae of both sexes at a higher level than those of other BminORs, BminOR24 was selected for further functional analyses. BminOR24/BminOrco expressed in Xenopus oocytes responded significantly to linalool. The identification of B. minax OR genes lays a foundation for further functional studies of OR genes, and functional characterization of BminOR24 provides insight for improving methods for controlling B. minax, a devastating pest insects.

Keywords: Bactrocera minax; Linalool; Odorant receptor; Transcriptome; Xenopus oocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyclic Monoterpenes / metabolism*
Amino Acid Sequence
Animals
Arthropod Antennae / physiology
Female
Insect Proteins / chemistry
Insect Proteins / genetics*
Insect Proteins / metabolism
Male
Phylogeny
Receptors, Odorant / chemistry
Receptors, Odorant / genetics*
Receptors, Odorant / metabolism
Sequence Alignment
Tephritidae / genetics*
Tephritidae / metabolism

Substances

Acyclic Monoterpenes
Insect Proteins
Receptors, Odorant
linalool