Enhanced effect of nano-monetite hydrosol on dentin remineralization and tubule occlusion

Shenglong Tan; Shangsi Chen; Yifan Wang; Feige Wu; Yufei Shi; Jianglin Wang; Yinyin Du; Shengmin Zhang

doi:10.1016/j.dental.2020.03.028

Enhanced effect of nano-monetite hydrosol on dentin remineralization and tubule occlusion

Dent Mater. 2020 Jun;36(6):816-825. doi: 10.1016/j.dental.2020.03.028. Epub 2020 Apr 28.

Authors

Shenglong Tan¹, Shangsi Chen¹, Yifan Wang¹, Feige Wu¹, Yufei Shi¹, Jianglin Wang¹, Yinyin Du¹, Shengmin Zhang²

Affiliations

¹ Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan 430074, China; Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
² Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan 430074, China; Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: smzhang@hust.edu.cn.

PMID: 32359849
DOI: 10.1016/j.dental.2020.03.028

Abstract

Objectives: We aim to investigate the dentin tubule occlusion and remineralization potential of a novel nano-monetite hydrosol (nMH).

Methods: First, nano-monetite hydrosol (nMH) was fabricated by homogeneous precipitation method. Then, the effectiveness of toothpaste with nMH on improving remineralization was evaluated by the measurement of tubule occluding ratio and acid-resistant stability compared with dentifrices comprising nano-hydroxyapatite hydrosol (nHH) and bioactive glass (BG). To explain this result, we studied the ions releasing and remineralization based on gelatin scaffold among nMH, nHH and BG. Finally, the cytotoxicity of these three minerals on Human dental pulp stem cells (HDPSCs) was evaluated.

Results: Processing for more than 7 days, the toothpaste containing nMH exhibited the significant remineralization potential and acid-resistant compared with two commercial de-sensitive dentifrices comprising nHH and BG. In addition, cytotoxicity test resulted that nMH has good cell compatibility to HDPSCs below extracts concentration of 3.12mg/mL.

Significance: Small size, the release of Ca²⁺ and PO₄^3- with high concentration, strongly binding on dental surface, and fast transformation to HAp, were all needed in the preparation of effective dentin tubule occluding biomaterials.

Keywords: Acid-resistant; Dentin tubules; Monetite hydrosol; Remineralization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dentin*
Glass
Humans
Microscopy, Electron, Scanning
Tooth Remineralization*
Toothpastes

Substances

Toothpastes