CPS1 expression and its prognostic significance in lung adenocarcinoma

Geting Wu; Zijin Zhao; Yuanliang Yan; Yangying Zhou; Jie Wei; Xi Chen; Wei Lin; Chunlin Ou; Jia Li; Xiang Wang; Kun Xiong; Jianhua Zhou; Zhijie Xu

doi:10.21037/atm.2020.02.146

CPS1 expression and its prognostic significance in lung adenocarcinoma

Ann Transl Med. 2020 Mar;8(6):341. doi: 10.21037/atm.2020.02.146.

Authors

Geting Wu¹, Zijin Zhao², Yuanliang Yan^{3

4}, Yangying Zhou⁵, Jie Wei^{3

4}, Xi Chen^{3

4}, Wei Lin¹, Chunlin Ou¹, Jia Li¹, Xiang Wang^{3

4}, Kun Xiong⁶, Jianhua Zhou¹, Zhijie Xu^{1

4}

Affiliations

¹ Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China.
² Department of Neurosurgery, Xiangya Hospital, Central South University, Institute of Skull Base Surgery and Neuro-oncology at Hunan, Changsha 410008, China.
³ Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China.
⁴ National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China.
⁵ Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China.
⁶ Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha 410013, China.

Abstract

Background: Studies have increasingly shown that carbamoyl phosphate synthetase 1 (CPS1) plays a vital role in the occurrence and development of human malignant disease. Unfortunately, the detailed function of CPS1 in the development and prognosis of lung cancer, especially lung adenocarcinoma (LADC), is still not fully understood. In this research, we performed a comprehensive bioinformatics analysis with respect to the function of CPS1 in human LADC.

Methods: Several biological databases including UALCAN, GEPIA and Oncomine were used to analyze the expression of CPS1 in LADC. Meanwhile, TCGA and GEO databases were utilized to analyze relevant clinical data. In addition, databases including Methsurv, etc., were used to analyze CPS1 methylation levels in LADC.

Results: The Oncomine platform, UALCAN and gene expression profiling interactive analysis (GEPIA) were used and revealed that the expression levels of CPS1 were significantly increased in LADC tissues. Furthermore, we analyzed the methylation level of CPS1 in LADC and found that cases with high levels of CPS1 showed hypomethylated CPS1. The clinical data from the Wanderer database, which is linked to The Cancer Genome Atlas (TCGA) database, demonstrated that the expression and methylation values of CPS1 were both significantly related to the clinical characteristics and prognosis of LADC. Through analysis of the dataset from the Gene Expression Omnibus (GEO) database, we found that the expression level of CPS1 was markedly downregulated in human A549 lung cancer cells treated with the chemotherapeutic drug motexafin gadolinium (MGd) in a time-dependent manner.

Conclusions: Our work indicated that CPS1 is upregulated in LADC samples and that CPS1 might be used as a potential biomarker for the diagnostic and prognostic evaluation of LADC. Determining the detailed biological function of CPS1 in LADC tissues will provide promising and insightful information for our further study.

Keywords: Carbamoyl phosphate synthetase 1 (CPS1); diagnosis; expression; lung adenocarcinoma; therapeutic target.