Modular and Versatile Trans-Encoded Genetic Switches

Avishek Paul; Eliza M Warszawik; Mark Loznik; Arnold J Boersma; Andreas Herrmann

doi:10.1002/anie.202001372

Modular and Versatile Trans-Encoded Genetic Switches

Angew Chem Int Ed Engl. 2020 Nov 9;59(46):20328-20332. doi: 10.1002/anie.202001372. Epub 2020 Jul 27.

Authors

Avishek Paul^{1

2}, Eliza M Warszawik¹, Mark Loznik^{1

2}, Arnold J Boersma^{2

3}, Andreas Herrmann^{1

2

3}

Affiliations

¹ Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
² DWI-Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52056, Aachen, Germany.
³ Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany.

Abstract

Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans-encoded and based on tRNA-mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA.

Keywords: RNA; RNA logic gates; RNA switches; synthetic biology; tRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression
Green Fluorescent Proteins / genetics
RNA, Bacterial / genetics*
Synthetic Biology

Substances

RNA, Bacterial
Green Fluorescent Proteins