Targeted imaging of integrins in cancer tissues using photocleavable Ru(ii) polypyridine complexes as mass-tags

Jiaying Han; Jing Sun; Shanshan Song; Leonie Beljaars; Geny M M Groothuis; Hjalmar Permentier; Rainer Bischoff; Gyorgy B Halmos; Cornelia J Verhoeven; Erika R Amstalden van Hove; Peter Horvatovich; Angela Casini

doi:10.1039/d0cc00774a

Targeted imaging of integrins in cancer tissues using photocleavable Ru(ii) polypyridine complexes as mass-tags

Chem Commun (Camb). 2020 Jun 4;56(44):5941-5944. doi: 10.1039/d0cc00774a. Epub 2020 Apr 29.

Authors

Jiaying Han¹, Jing Sun, Shanshan Song, Leonie Beljaars, Geny M M Groothuis, Hjalmar Permentier, Rainer Bischoff, Gyorgy B Halmos, Cornelia J Verhoeven, Erika R Amstalden van Hove, Peter Horvatovich, Angela Casini

Affiliation

¹ Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands. p.l.horvatovich@rug.nl.

PMID: 32347235
DOI: 10.1039/d0cc00774a

Abstract

Targeted epitope-based mass spectrometry imaging (MSI) utilizes laser cleavable mass-tags bound to targeting moieties for detecting proteins in tissue sections. Our work constitutes the first proof-of-concept of a novel laser desorption ionization (LDI)-MSI strategy using photocleavable Ru(ii) polypyridine complexes as mass-tags for imaging of integrins αvβ3 in human cancer tissues.

MeSH terms

Head and Neck Neoplasms / metabolism*
Humans
Integrin alphaVbeta3 / metabolism*
Mass Spectrometry / methods
Peptides, Cyclic / chemistry
Peptides, Cyclic / pharmacology*
Pyridines / chemistry
Pyridines / pharmacology*
Ruthenium / chemistry
Ruthenium / pharmacology*

Substances

Integrin alphaVbeta3
Peptides, Cyclic
Pyridines
cyclic (arginyl-glycyl-aspartyl-phenylalanyl-lysyl)
Ruthenium